Fig. 2.

FRET study of the formation of A₁₄₁₁/OliP and the light-triggered release of A₁₄₁₁. (A) Schema of the formation of A₁₄₁₁/OliP FRET pairs through hybridization of fluorophore (F, Cy3)-labeled A₁₄₁₁ to quencher (Q, Iowa Black RQ)-labeled OliP. (B) Schema of light-triggered release of Cy3-A₁₄₁₁ with inhibition of the FRET (i.e., increased fluorescence). (C) Fluorescence spectrum of Cy3-A₁₄₁₁ before and after hybridization with quencher-labeled OliP. (D) Time course of the fluorescence spectrum of A₁₄₁₁/OliP following dehybridization under continuous irradiation (365 nm light at 5 mW/cm²).