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APE1 is required for efficient Sμ–Sα synapse formation during CSR. (A) Scheme of long-range interactions between Sμ–Sα elements in the IgH locus before and after AID activation. (B) Representative gel picture of the 3C assay detecting Sμ–Sα interaction in the three cell lines stimulated (or not) with CIT for 24 h. GAPDH was amplified as loading control. (C) ChIP and quantitative PCR analysis for Ku80 in cells stimulated (or not) with CIT for 24 h. Data are represented as mean ± SD.
