Table 2.
Fusion protein | koff hFcRn, s−1* | koff mFcRn, s−1† | In vivo half-life, h | ||
−HSA | +HSA | −MSA | +MSA | ||
ZFcRn_4-ABD-ZTaq | 0.2 | 0.05 | ---‡ | 0.006 | 73 |
ZFcRn_16-ABD-ZTaq | 0.04 | 0.02 | 0.3 | 0.006 | 91 |
ZFcRn_2-ABD-ZTaq | 0.03 | 0.02 | 0.2 | 0.006 | 61 |
ZTaq-ABD | NB | NB | NB | NB | 33 |
NB, not binding.
The analysis was performed in the presence or absence of HSA.
The analysis was performed in the presence or absence of MSA.
The off-rate was too fast to be measured with the current setup.