Table 2.

Analysis of the apparent off-rate and in vivo half-lives of the fusion proteins

Fusion protein	koff hFcRn, s−1*		koff mFcRn, s−1†		In vivo half-life, h
	−HSA	+HSA	−MSA	+MSA
ZFcRn_4-ABD-ZTaq	0.2	0.05	---‡	0.006	73
ZFcRn_16-ABD-ZTaq	0.04	0.02	0.3	0.006	91
ZFcRn_2-ABD-ZTaq	0.03	0.02	0.2	0.006	61
ZTaq-ABD	NB	NB	NB	NB	33

NB, not binding.

^*The analysis was performed in the presence or absence of HSA.

^†The analysis was performed in the presence or absence of MSA.

^‡The off-rate was too fast to be measured with the current setup.