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. 2014 Nov 17;111(48):17104–17109. doi: 10.1073/pnas.1418637111

Fig. 2.

Fig. 2.

NanoFlares detect mesenchymal markers in EMT model cell lines. (A) NanoFlares detect E-cadherin and Twist mRNA in model mammary cell lines, HMLE, and HMLE-Twist. Results represent flow-cytometric analysis of cells treated with NanoFlares (100 pM) for 16 h. (B) NanoFlares detect EMT markers in metastatic breast cancer cell line MDA-MB-231. NanoFlares were designed for mesenchymal markers (Vimentin and Fibronectin), an epithelial marker (E-cadherin), and a nontargeting Scrambled Control NanoFlare. Results represent flow-cytometric analysis of cells treated with NanoFlares (100 pM) for 8 h.