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. 2014 Nov 17;111(48):17170–17175. doi: 10.1073/pnas.1413118111

Fig. 1.

Fig. 1.

Comparison of the oligomeric states and collisional activation required to release MscL from detergent micelles in the gas phase. (A) Ion mobility spectrum (Upper) and the excerpt of the mass spectrum in the region where monomeric MscL is observed when released from DDM micelles. Release of MscL from DDM micelles requires high collision energies, which are already enough to disrupt the MscL pentamer, yielding only monomeric subunits. (B) Ion mobility spectrum (Upper) and complementary mass spectrum (Lower) of pentameric MscL released from Triton X-100 micelles. Colored lines indicate calculated m/z values for pentameric MscL with and without several detergent molecules attached (average mass 625 Da for Triton X-100 used). (C) Ion mobility spectrum (Upper) and the excerpt of the mass spectrum in the region where monomeric and dimeric MscL is observed when released from LDAO micelles.