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. Author manuscript; available in PMC: 2014 Dec 9.

Published in final edited form as: J Biol Rhythms. 2014 Aug;29(4):257–276. doi: 10.1177/0748730414543141

Alterations in bdh1 mRNA in CBK (microarray [i], RT-PCR [ii]) and CCM (RT-PCR [iii]) hearts, compared to littermate controls (A). Lack of bdh1 mRNA oscillations in serum shocked adult rat cardiomyocytes (i.e., challenged with 50% serum for 2 hours [shaded area]; B). Lack of diurnal variations in BDH1 protein levels in control hearts (Ci). Decreased BDH1 protein levels in CBK (Cii) and CCM (Ciii) hearts at ZT6. Decreased BDH activity in CBK (Di) and CCM (Dii) hearts at ZT6. Decreased β-hydroxybutyrate oxidation in ex vivo perfused CBK (Ei) and CCM (Eii) hearts at ZT6. Mice were housed in a 12-hr light:12-hr dark cycle (lights on at ZT0). Hearts were isolated from 12 week old male CBK, CCM, and age-matched littermate controls. Note that ZT0 and ZT24 are identical data. Values are expressed as mean ± SEM (n=3–7; sample size range varies dependent on the parameter investigated). *, p<0.05 for CBK/CCM versus littermate control at a distinct ZT (post-hoc pair-wise comparison).

Alterations in bdh1 mRNA in CBK (microarray [i], RT-PCR [ii]) and CCM (RT-PCR [iii]) hearts, compared to littermate controls (A). Lack of bdh1 mRNA oscillations in serum shocked adult rat cardiomyocytes (i.e., challenged with 50% serum for 2 hours [shaded area]; B). Lack of diurnal variations in BDH1 protein levels in control hearts (Ci). Decreased BDH1 protein levels in CBK (Cii) and CCM (Ciii) hearts at ZT6. Decreased BDH activity in CBK (Di) and CCM (Dii) hearts at ZT6. Decreased β-hydroxybutyrate oxidation in ex vivo perfused CBK (Ei) and CCM (Eii) hearts at ZT6. Mice were housed in a 12-hr light:12-hr dark cycle (lights on at ZT0). Hearts were isolated from 12 week old male CBK, CCM, and age-matched littermate controls. Note that ZT0 and ZT24 are identical data. Values are expressed as mean ± SEM (n=3–7; sample size range varies dependent on the parameter investigated). *, p<0.05 for CBK/CCM versus littermate control at a distinct ZT (post-hoc pair-wise comparison).