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. 1998 Dec 14;28(8):2549–2558. doi: 10.1002/(SICI)1521-4141(199808)28:08<2549::AID-IMMU2549>3.0.CO;2-O

Figure 5.

Figure 5

Immature self-reactive B cells from mHEL/anti-HEL double Tg donors respond to T cell help in vitro but not in vivo. (a–d) FACS analysis of B220+ spleen cells obtained from mHEL Tg recipients 1 (a, b) and 3 (c, d) days after transfer of immature bone marrow-derived B cells from mHEL/anti-HEL double Tg donors. Undivided immature B cells (dotted rectangles) have down-regulated surface Ig in response to mHEL. (e, f) In vitro response of immature B cells from mHEL/anti-HEL Tg donors co-cultured with activated T cells and either mHEL, sHEL or no HEL. Cells were harvested 2, 4, or 7 days after initiation of culture and HEL-binding B cells were counted by flow cytometry and expressed per 105 live cells.