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Effects of mutations at branch A to opsin A2_Y277F/N294K
| Template | Mutants | λmax ± SD (nm) | Δλ |
|---|---|---|---|
| A2_Y277F/N294K | 539 ± 1.2 | ||
| A2_Y277F/N294K/N33S/A35S | 538 ± 2.5 | −1 | |
| A2_Y277F/N294K/D213Y | Failed | NA | |
| A2_Y277F/N294K/K217Q | 538 ± 2.2 | −1 | |
| A2_Y277F/N294K/N33S/A35S/K217Q | 540 ± 2.3 | +1 |
