Table 1. Differentially regulated proteins involved in essential amino-acid pathways of Buchnera-LSR1.
Protein name | Pathway | ANOVA P-valuea | Fold change | Transcription factorsb | qRT-PCRc |
---|---|---|---|---|---|
Upregulated in bacteriocytes | |||||
HisG | Histidine | 0.0089 | 1.8 | DksA | NS |
HisC | Histidine | 0.003 | 1.6 | DksA | NS |
IlvH | Branched-chain amino acids | 0.0017 | 1.5 | Hns | — |
Upregulated in embryos | |||||
AroK | Aromatic amino acids | 0.0063 | 1.6 | *2 | — |
SerC | Sulfate reduction | 0.0124 | 1.7 | *2 | — |
ThrB | Threonine | 0.0058 | 1.7 | DksA | NS |
ArgA | Arginine | 0.0004 | 2.1 | *1 | NS |
Fba | Glycolysis | 0.0087 | 2.2 | *2 | NS |
LysA | Lysine | 0.0002 | 2.8 | *2 | NS |
Abbreviations: ANOVA, analysis of variance; LFQ, label-free quantification; NS, not significantly different between treatments; qRT-PCR, quantitative reverse transcriptase-PCR; ‘—', not tested.
LFQ analysis of Buchnera proteins between two Buchnera life stages.
Transcription factors conserved in Buchnera that are critical for E.coli regulation. *N indicates the number of E. coli transcription factors that are missing in Buchnera.
Quantitative reverse transcriptase-PCR was conducted between life stage treatments for the specific gene with differential protein expression.