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. 2014 May 23;8(12):2397–2410. doi: 10.1038/ismej.2014.81

Figure 6.

Figure 6

Changes in the relative frequencies of 16S rRNA gene sequence reads related to the three most abundant and labeled phylotypes of the archaeal ammonia-oxidizing (a), nitrite-oxidizing (b) and methane-oxidizing (c) community in fractionated DNA from microcosms regularly amended with low (15 μg NH4+-N per g dry soil added initially; 7.5 μg NH4+-N per g dry soil on days 7, 14 and 21) and high (150 μg NH4+-N per g dry soil added initially; 75 μg NH4+-N per g dry soil on days 7, 14 and 21) levels of ammonium sampled on days 14 and 28. Microcosms were incubated with and without 1% methane in the headspace for the last 14 days of incubation. The relative frequencies are expressed as the percentage of 16S rRNA genes from the respective OTU to total 16S rRNA genes of each DNA fraction. Data plotted are mean values and s.e. from DNA obtained from gradient fractions of duplicate nonlabeled 12C (open symbols) and labeled 13C (closed symbols) microcosms.