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. Author manuscript; available in PMC: 2014 Dec 9.
Published in final edited form as: J Immunol. 2011 Jul 22;187(5):2677–2687. doi: 10.4049/jimmunol.1002711

Figure 2. The effects of nicotine treatment of mice with oxazolone- and TNBS-induced colitides on the peripheral and colonic populations of T lymphocytes.

Figure 2

The FCM assays with T cell marker antibodies were performed using splenocytes and LPMCs freshly isolated from intact (control) and nicotine-treated and untreated mice with experimental colitides at the end of nicotine treatments, as detailed in the Materials and Methods section. The cells were triple stained for CD3, CD4 and CD8 and examined by flow cytometry. The contour plots were generated after gating on lymphocytes (by forward and side scatter) for CD3 staining or gating on CD3 T cells during the analyses of CD4/CD8 staining. Shown on the graphs are representative dot blots for the CD3 T cell population of spleen MCs (A) and the CD4 and CD8 T cell subpopulations of CD3 T cells from spleen (B) and colon (C). Note: treatment with nicotine decreased the percentage of spleen CD3 and CD4 T cells to the normal levels in mice with oxazolone colitis, and further elevated the percentage of CD3 T cells without altering the already elevated numbers of spleen CD4 T cells in mice with TNBS colitis.