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. Author manuscript; available in PMC: 2014 Dec 9.
Published in final edited form as: J Immunol. 2011 Jul 22;187(5):2677–2687. doi: 10.4049/jimmunol.1002711

Figure 4. The nicotinergic effects on the development of Tregs from the naïve CD4+CD62L+ T cells stimulated with anti-CD3/CD28 antibodies.

Figure 4

The enriched naïve CD4+CD62L+ T cells isolated from the spleens of intact BALB/c mice were cultured in growth medium alone (condition “1”) or stimulated with anti-CD3 and anti-CD28 antibodies in the absence (condition “2”) or presence of nicotine (Nic; condition “3”), 10 ng/ml of IL-4, 100 μM of nicotine plus 10 ng/ml of IL-4 or IL-12 or 100 μM of nicotine plus 10 ng/ml of IL-12 as detailed in Materials and Methods. After 5 days in culture, the expression of Foxp3 protein was analyzed by immunoblotting (A) and FCM (B–D). The graph in panel “A” demonstrates changes of Foxp3 concentration after its normalization for the level of the house keeping protein β-actin. In panels “B–D”, the changes in Foxp3 expression are shown as an overlay histogram representing different treatment conditions.