Table 1. Differential expression of ATF4 and its selected transcriptional targets in HCT116 cells after mitochondrial ETC complex III inhibition with myxothiazol for 5 h (M5), 13 h (M13), 17 h (M17) and 13 h in the presence of uridine (MU13).
| Gene ID | Gene name |
mRNA fold changesa |
|||
|---|---|---|---|---|---|
| M5 | M13 | M17 | MU13 | ||
| Atf4 | activating transcription factor 4 (tax- responsive enhancer element B67) | 2.46 | 0.43 | 0.24 | 1.76 |
| Trib3 | tribbles homolog 3 (Drosophila) | 5.41 | NC | 0.55 | 3.24 |
| Ddit3 | DNA-damage-inducible transcript 3, C/EBP homologous protein (CHOP) | 5.53 | 2.07 | NC | 2.77 |
| Chac1 | ChaC, cation transport regulator homolog 1 (E. coli) | 7,9 | NC | 0,28 | 4.98 |
| Slc7a11 | solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 | 2.41 | NC | 0.24 | 4.76 |
| Asns | asparagine synthetase | 3.56 | 2.98 | 1.53 | 3.05 |
Abbreviation: NC, no change.
a
Fold change in mRNA levels according to mRNA-seq data is presented (FDR<0.05).