Figure 2.

VSOR Cl⁻ channel blockers rescue tunicamycin-induced ER stress in cardiomyocytes. Cardiomyocytes were treated with Tm (100 ng/ml) in the presence or absence of DIDS or DCPIB for 24 h. (a) Representative images of immunostaining for GRP78 (Green). Nuclei were labeled with DAPI; n=5 for each group. (b) qRT-PCR assay for XBP1S expression. β-Actin served as a loading control. *P<0.05 versus ctrl; **P<0.05 versus Tm, n=5. (c) Western blot analysis and quantitative assay for ATF4, p-eIF2α and CHOP protein expressions. β-Actin served as a loading control. *P<0.05 versus ctrl; **P<0.05 versus Tm, n=5. (d) Expression of CHOP by immunofluorescence (Green). Nuclei were counterstained with DAPI; n=5 for each group