Figure 3.

Protective effect of VSOR Cl⁻ channel inhibitors on tunicamycin-induced cardiomyocyte death. Cardiomyocytes were treated with Tm (100 ng/ml) in the presence or absence of DIDS or DCPIB for 72 h. (a) Protective effect of DIDS and DCPIB on Tm-induced cardiomyocyte viability measured by MTT-assay, respecitvely. *P<0.05 versus ctrl; **P<0.05 versus Tm, n=5. (b) TUNEL staining of cardiomyocytes. Quantitative analysis of TUNEL-positive cardiomyocytes after Tm treatment with or without DIDS (75 μM) and DCPIB (2 μM) for 72 h. *P<0.05 versus ctrl; **P<0.05 versus Tm, n=5. (c) Activity analysis of caspase-3. *P<0.05 versus ctrl; **P<0.05 versus Tm, n=5