Figure 4.

The CHOP-Wnt pathway in VSOR Cl⁻ channel blockers mediated protective role. (a) Western blot analysis and quantitative assay of both cytosol and nuclear expression of β-catenin in cardiomyocytes after Tm treatment with or without DIDS (75 μM) and DCPIB (2 μM) for 24 h; n=5 for each group. (b) Topflash and pTK-Rennila luciferase vector were transfected with siNC or siCHOP for 36 h in H9C2 cells. *P<0.05 versus ctrl, n=5. (c) H9C2 cells were transfected with Topflash and pTK-Rennila luciferase vector with or without siCHOP. After 12 h transfection, cells were additionally treated with tunicamycin with or without DIDS, DCPIB for 24 h, respectively. *P<0.05 versus ctrl; **P<0.05 versus Tm, n=5. (d–f) Topflash and pTK-Rennila luciferase vector with or without siCHOP were transfected for 12 h in H9C2 cells. After 12 h transfection, cells were additionally treated as indicated for 24 h, respectively. (d) *P<0.05 versus Tm+siNC, n=5; (e) *P<0.05 versus Tm; **P<0.05 versus Tm+DIDS; ^#P<0.05 versus Tm+DCPIB, n=5; (f) *P<0.05 versus Tm+siCHOP; **P<0.05 versus Tm+siCHOP+DIDS; ^#P<0.05 versus Tm+siCHOP+DCPIB, n=5. (g–i) Cardiomyocytes were transfected with or without siCHOP for 24 h, and then cells were additionally treated as indicated for 72 h. Cell viability was measured by the MTT-assay in cardiomyocytes. (g) *P<0.05 versus Tm+siNC, n=5; (h) *P<0.05 versus Tm; **P<0.05 versus Tm+DIDS; ^#P<0.05 versus Tm+DCPIB, n=5; (i) *P<0.05 versus Tm+siCHOP; **P<0.05 versus Tm+siCHOP+DIDS; ^#P<0.05 versus Tm+siCHOP+DCPIB, n=5