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Tunicamycin induced ROS production in vitro and in vivo. (a) Effect of Tm (100 ng/ml) for 48 h on the ROS level of cardiomyocytes monitored by dihydroethidine (DHE) staining (Red). Nuclei were counterstained with DAPI; n=5 for each group. (b) Increased ROS accumulation in tunicamycin (3 mg/kg, 48 h, i.p.)-exposed myocardium was revealed by DHE staining (Red). Nuclei were counterstained with DAPI; n=5 for each group
