Figure 6.

ROS induce VSOR Cl⁻ currents in cardiomyocytes. (a) Background Cl⁻ currents recorded under isosmotic solution (Ctrl). H₂O₂ (500 μM)-induced Cl⁻ currents exhibiting phenotypic properties of I_Cl,Vol (H₂O₂). H₂O₂-induced VSOR Cl⁻ currents were inhibited by adding DIDS (500 μM); n=5 for each group. (b) Corresponding current-voltage (I-V) relationship for the mean current densities of isosmotic (▴), H₂O₂ (▪) and H₂O₂ with DIDS (●) conditions. (c) Current densities at +100 mV from B. *P<0.05 versus Ctrl; **P<0.05 versus H₂O₂, n=5. (d) Negligible background Cl⁻ currents recorded under isosmotic solution (Ctrl). H₂O₂ (500 μM)-induced Cl⁻ currents exhibiting representative properties of VSOR Cl⁻ currents (H₂O₂). H₂O₂-induced VSOR Cl⁻ currents were inhibited by adding DCPIB (10 μM). n=5 for each group. (e) Corresponding current-voltage (I-V) relationship for the mean current densities of Ctrl (▴), Tm (▪) and Tm with DIDS (●) conditions. (f) Current densities at +100 mV from (e). *P<0.05 versus Ctrl; **P<0.05 versus H₂O₂, n=5