Figure 4.

PKCη expression has opposing effects on IL-6 and IL-8 secretions. MCF-7 clones expressing sh-PKCη (sh η 3-5, sh η 2-2 and sh η 4-2) or scrambled control cells (sh scr 5-3) were treated with 150 μM H₂O₂ or 400 μM etoposide for 2 h. Fresh growth medium was added for 96 h. Medium was collected and assayed by ELISA for (a, b) IL-6 and (c, d) IL-8 protein levels as described in Materials and Methods. Values are represented as pg/ml, calculated from standard curves of at least three separate experiments performed in triplicate. Error bars represent the S.D.; two-tailed, unpaired sample t-test statistical analysis is shown: *P≤0.05, **P≤0.001 and ***^/$$$P≤0.0001 (* represents statistical significance for each non-treated and treated clone and ^$ represents statistical significance between different clones)