Figure 3.

Resveratrol induces a transient activation of the DNA-damage response in vivo. (a) Effects of daily oral administrations of 200 mg/kg of RSV on the growth of SW620 tumours in vivo. SW620 cells were subcutaneously injected at day 0 to nude mice and the treatments with RSV or with the vehicle (Ctl) began at day 2. Data are medians of tumour volumes of 9–12 mice per group±the first and third quartile. Statistical analyses were performed by the Mann–Whitney's test and are shown as significant with P<0.05 (*) and P<0.01 (**). (b, c) Levels of expression and phosphorylation of the components of the DNA-damage response were analysed by immunoblotting using the specified antibodies. Analyses were performed on SW620 tumours extracts collected from five mice per group after 11 (b) and 15 days (c) of daily treatments with 200 mg/kg of RSV (R200) and compared to control tumours (Ctl). Quantifications by densitometry are plotted as medians±the first and third quartile. Statistical analyses were performed by the Mann–Whitney's test and are shown as significant with P<0.05 (*) and P<0.01 (**)