Figure 6.

Resveratrol promotes the phosphorylation of the DNA-damage marker histone H2AX. (a) Flow cytometric analyses of γH2AX/PI staining on PROb and SW620 cells after 1 day of treatment (d1) with 30 μM of RSV (R30) or mock-treated (Ctl); the percentages represent the amounts of γH2AX positive cells. (b) Microscopic analysis of γH2AX immunostainings of PROb cells grown on coverslips treated with R30 during 1 (d1) or 3 days (d3). Arrows show macronucleated cells (M) and apoptotic ones (A); bars, on the left 100 μm, on the right 20 μm. (c, d) Time course of expression and phosphorylation of the components of the ATR, ATM and DNA-PK pathways were analysed by immunoblotting using the specified antibodies. PROb and SW620 cells as well as their resistant counterpart, R²PROb and R²SW620 populations, were treated with R30 for indicated times in days (d) and compared with the untreated cells (Ctl)