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. 2014 Nov 27;5(11):e1540. doi: 10.1038/cddis.2014.504

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International Licence. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons licence, users will need to obtain permission from the licence holder to reproduce the material. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0

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Combination of bortezomib and citreoviridin exhibited an additive effect on cytotoxicity. (a) Real-time cell growth of MCF7 cells was measured after drug treatment (x axis) at hourly intervals for 48 h by using an RTCA system and expressed as a normalized cell index (y axis). Cells were treated with 0.1 or 0.2 μM citreoviridin (Citreo) in the presence or absence of 10 nM bortezomib (Btz). (b) Colony-formation assays were assessed through anchorage-dependent (left, ADG) or anchorage-independent (right, AIG) procedures, as described in the Materials and Methods section. Cells were treated with DMSO, 0.1 μM citreoviridin (Citreo), 10 nM bortezomib (Btz), or a combination of 0.1 μM Citreo and 10 nM Btz (Citreo+Btz). Colonies were grown in six-well plates and then stained with crystal violet. (c) The numbers of colonies of ADG in each well and AIG in each field per well were counted. Asterisks indicate significant differences between the control and treated group from three independent experiments (*P<0.01; **P<0.001)