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. 2014 Nov 27;5(11):e1545. doi: 10.1038/cddis.2014.511

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International Licence. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons licence, users will need to obtain permission from the licence holder to reproduce the material. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0

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Rapamycin treatment stimulates calcineurin activity. (a) Histogram of calcineurin activity obtained by monitoring ³²P release from radiolabeled purified RII substrate in axotomized precerebellar nuclei of saline- and rapamycin-treated Ctrl (Ctrl+S, Ctrl+R12h, and Ctrl+R24h) and saline- and rapamycin-treated HCb (HCb12h+S, HCb12h+R, HCb24h+S, and HCb24h+R) mice at 12 and 24 h. Data are expressed as mean (Ctrl+S percentage)±S.D. (*P<0.05, n=5). (b) Confocal images of RCAN1 staining of precerebellar nuclei of Ctrl+S, Ctrl+R, HCb+S, and HCb+R mice at 24 h. (c) Densitometric graph of RCAN1 staining in Ctrl+S, Ctrl+R24h, HCb24h+S, and HCb24h+R mice. Data are expressed as mean±S.D. (***P<0.001, n=6 mice per group, N=180 cells per group). (d) Representative immunoblots and densitometric graph of RCAN1 levels (Ctrl+S percentage) in precerebellar nuclei of Ctrl+S, Ctrl+R24h, HCb24h+S, and HCb24h+R mice at 24 h. Actin was used as loading control. Data are expressed as mean ±S.D. (*P<0.05, **P<0.01, n=5)