Table 1.

Fold change of selected genes from Innate and Adaptive Immune Responses PCR Array¹

		PCR Array		qRT-PCR Validation
Gene Name	Symbol	Fold change^a	P-value	Fold change (± SE)^a	P-value
Heme oxygenase (decycling) 1	HMOX1	2.9	0.01	4.159 ± 0.524	<0.001
Interleukin 6 (interferon, beta 2)	IL6	9.7	2.2 E-5	46.555 ± 15.197	0.016
Lipopolysaccharide binding protein	LBP	3.5	0.018	2.301 ± 0.171	0.001
Triggering receptor expressed on myeloid cells 1	TREM1	115.2	0.0009	7.079 ±0.162	<0.001
Prostaglandin-endoperoxide synthase 2^b	PTGS2	−1.2	0.59	7.677 ± 2.128	0.018

HTR-8 cells were treated with DMSO (0.1 % v/v; solvent control) or 10 μM TBBPA for 24 h. The Innate and Adaptive Immune Responses PCR Array (SABiosciences; Valencia, CA) was used to explore changes in gene expression as described in the “Materials and Methods” section. Fold changes and p-values are shown for the genes with significant changes compared to the solvent control.

Mean fold change relative to the solvent control. PCR Array: n= 4 experiments, qRT-PCR Validation: n=3 experiments. Each experiment was performed in triplicate.

Although the change was not statistically significant in the array, gene expression was assessed by qRT-PCR because of this gene’s pertinence to human pregnancy.