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. Author manuscript; available in PMC: 2014 Dec 9.
Published in final edited form as: Oncogene. 2012 Apr 23;32(9):1135–1143. doi: 10.1038/onc.2012.135

Figure 5. Rab6 vesicle velocity is regulated by a Merlin-Rac pathway.

Figure 5

The velocities of anterograde Rab6-mStrawberry exocytic vesicles in transfected SC4 cells displayed as both frequency histograms (left) and a bar graph of mean velocities with the 95% confidence interval (right). A) SC4 cells co-transfected with Rab6-mStrawberry and either a merlin expressing plasmid or empty vector. B) SC4 cells treated for 2–3 hours with 100 μM of the Rac inhibitor NSC23766. C) SC4 cells co-transfected with Rab6-mStrawberry and either an active, fast cycling F28L-Rac mutant expressing plasmid or empty vector. D) SC4 cells co-transfected with Rab6-mStrawberry and either a constitutively GTP-bound Q61L-Rac mutant expressing plasmid or empty vector. E) SC4 cells treated for 2–3 hours with 100 nM of the MLK inhibitor CEP11004. F) SC4 cells treated for 2–3 hours with 20 μM of the p38SAPK inhibitor SB203580.