Figure 3. 3-MA prevents LC3 accumulation and reduces cediranib/quinacrine efficacy.

A) Representative LC3 western blots obtained from 4C8 cells grown under hypoxic (0.5% O₂) conditions for 24 hrs while exposed to a combination of cediranib (2 µM) and quinacrine (1.5 µM), either with or without 2 mM 3-MA. B) Results from MTS cell viability assays showing mean 4C8 cell viability in response to treatment, normalized to untreated samples. Assays were performed after cells were exposed to treatment for 72 hours, under hypoxic (0.5% O₂) conditions. Results are mean ± SEM of three independent experiments, performed in triplicate. Significant differences between tumor cell viability results obtained with and without 3-MA are denoted by * (p<0.05).