Table 1.

Effect of vital dyes on retinal pigmented epithelial cell viability, at different dye concentration and exposure times

Exposure time	Concentrations (mg/ml)
	Dyes	1 (Mean ± SD)	0,5 (Mean ± SD)	0,25 (Mean ± SD)	0,05 (Mean ± SD)	0,005 (Mean ± SD)
	BSS	100 ± 0	100 ± 0	100 ± 0	100 ± 0	100 ± 0
	BriB	64.8 ± 3.6**	77.4 ± 4.5**	94.4 ± 4.9	94.9 ± 6.2	98.2 ± 6.5
	ICG	43.1 ± 3.3**	51.6 ± 3.5**	55.2 ± 2.7**	61.9 ± 4.2**	66.3 ± 3.9**
	IfCG	56.5 ± 3.5**	62.0 ± 2.9**	68.1 ± 2.9**	74.3 ± 3**	79.2 ± 3.5**
	LG	92.7 ± 6.8	93.6 ± 4.3	96.9 ± 4.2	97.7 ± 4.8	101.2 ± 5.1
	FG	69.2 ± 5.1**	69.8 ± 8.3**	77.4 ± 4.8**	79.7 ± 4.6**	80.1 ± 3.4**
3 minutes	IC	47.3 ± 4.9**	53.0 ± 3.5**	56.4 ± 3.5**	59.7 ± 4.5**	66.6 ± 3.5**
	BroB	49.6 ± 3.1**	57.2 ± 3.7**	61.4 ± 2.4**	62.1 ± 4.9**	66.6 ± 5.3**
	EB	50.4 ± 3.6**	58.7 ± 6.3**	62.6 ± 7.2**	67.3 ± 8.1**	61.9 ± 5.3**
	CR	55.8 ± 3.1**	64.3 ± 4.1**	73.7 ± 3.4**	89.1 ± 5	93.9 ± 6.7
	BSS	100 ± 0	100 ± 0	100 ± 0	100 ± 0	100 ± 0
	BriB	62.3 ± 3.1**	72.8 ± 5.1**	81.6 ± 6.1**	87.4 ± 8.3*	94.2 ± 9.3
	ICG	33 ± 4.6**	31.4 ± 3.3**	33.5 ± 3.3**	37.2 ± 3.6**	37.2 ± 5**
	IfCG	52.4 ± 6.7**	52.3 ± 7.8**	54.6 ± 7.7**	53.4 ± 8**	48.5 ± 9.1**
	LG	39.4 ± 2.6**	44.9 ± 4.5**	47.1 ± 2.8**	45.5 ± 3.3**	46.7 ± 4**
	FG	46.3 ± 3.7**	51.1 ± 2.4**	44 ± 4.3**	41.3 ± 1.5**	56.1 ± 2**
30 minutes	IC	42.7 ± 2.4**	44.3 ± 3.3**	49.2 ± 2**	50.4 ± 3.1**	53.7 ± 3.1**
	BroB	37.2 ± 5.1**	51.6 ± 2.5**	56.9 ± 4.2**	57.3 ± 6.2**	60.4 ± 2**
	EB	39.9 ± 6.2**	52.2 ± 7**	55.4 ± 3.2**	60 ± 2.5**	58.6 ± 7.5**
	CR	39.9 ± 4.1**	42.5 ± 2**	49.9 ± 4.2**	52.3 ± 4**	59.9 ± 3.3**

Analysis of variance (ANOVA) –Tukey test

Observations:

I– Lower the values higher the dye toxicity.

II– Values are means of at least 3 independent experiments.

III – In regard to the P values presented in this table, there was a significant difference between the dyes with all concentrations and exposure times (* P < 0.05, ** P < 0.01) when compared with non-treated control cells