Figure 3. Effect of the peptide ANXA1_2–26 and antagonist Boc2 on the proliferation of Hep-2 cells.

Treatment with ANXA1_2–26 reduced the cellular growth. The antagonist Boc2 partially inhibited the anti-proliferative effect of ANXA1_2–26. The cells treated with Boc2 alone showed a level of proliferation similar to that of the control. The Hep-2 cells were seeded in MEM-Earle medium at a density of 2×10⁶ cells in 75-cm² culture flask, and then were incubated with serum-free medium 24 hours prior to the addition of ANXA1_2–26 (1 µM), ANXA1_2–26 (1 µM)+Boc (10 µM) or Boc (10 µM) alone. All of the experiments were performed in triplicate to confirm the results. Data are expressed as the mean ± SEM of the cell number ×10⁶. ** P<0.01 and *** P<0.001 vs. control, ## P<0.01 and ### P<0.001 vs. ANXA1_2–26+Boc.