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. 2014 Dec 9;9(12):e114318. doi: 10.1371/journal.pone.0114318

Figure 1. Outline of the procedure for isolating progenitor cells from muscle.

Figure 1

Figures 1A and 1B. Previously used procedure that entailed a 2 hour adherence to tissue culture polystyrene (TCPS) following by a 2 week (wk) outgrowth in tissue culture media followed by a flow cytometry analysis. Figures 1A and 1C. Procedure used in this study. A single cell suspension was used directly for flow cytometry analysis and cell sorting.