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. 2014 Dec 9;9(12):e114318. doi: 10.1371/journal.pone.0114318

Figure 3. Analysis of CD105, CD90 and CD73 positive cells in a single cell suspension from traumatized muscle.

Figure 3

A. single cell suspension (Figure 1C) from collagenase digested traumatized muscle tissue was processed for flow cytometry using fluorescent-conjugated antibodies against the cell surface markers indicated in the figure. The gated regions, indicated by a red circle within the plot, contain the positive cells. B. For each marker the bar graph reflects the number of positive cells isolated (mean ± SEM, n = 12) as in Figure 1B (2 Hr Adherence, 2 Wk outgrowth) or as in Figure 1C (Initial Population). Significant difference is noted (*) between CD73 and CD105 in the initial population and the other conditions (Student's t-test, p≤0.01).