Figure 5. Growth characteristics of CD29, CD31, CD34, CD56 and CD91 positive cells.
Flourescent-conjugated antibodies to CD29, CD31, CD34, CD56 and CD91 were used to separately sort and subculture cells form collagenase 2 digested human muscle. The cells were seeded at 1×105 cells per well in 6-well plates on day 0 and cultured for an additional two weeks. The graph indicates the cell number on the indicated days (mean ± SEM, n = 8). A significant difference is noted (*) between cell growth number for replicates between 10 and 15 days of growth for all five cell populations (Mann Whitney U test, p≤0.01).