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. 2014 Dec 9;9(12):e114318. doi: 10.1371/journal.pone.0114318

Figure 6. Migration characteristics of CD29, CD31, CD34, CD56 and CD91 positive cells.

Figure 6

Fluorescent-conjugated antibodies to CD29, CD31, CD34, CD56 and CD91 were used to separately sort and subculture cells from collagenase 2 digested human muscle tissue. The cells were seeded into wells of a Gap-Closure Assay plate. When the wells were confluent the circular plug was removed to allow the cells to migrate into the space. The graph indicates the % closure of the circular space at the indicated times (mean ± SEM, n = 8). A significant difference is noted (*) in migration rates between CD56 and CD31 compared to CD29, CD34 and CD91, between time points of 18 to 36 hours (Mann-Whitney U Test, p≤0.01). Scale bar  = 100 µm.