Figure 9. Endothelial cell differentiation of CD29+ and CD34+ cells.

Flourescent-conjugated antibodies to CD29, CD31, CD34, CD56 and CD91 were used to separately sort and subculture cells form collagenase 2 digested human muscle. The cells were cultured to confluence then were cultured for an additional two weeks in either vascular endothelial cell differentiation media (VEM) or regular growth media (GM). The cultures were fixed and processed for immunofluorescence microscopy using antibodies to VE-cadherin, Tie2 and CD31. Also shown is a representative phase contrast image of each cell type. Scale bar  = 50 µm.