Figure 2. Rapid RhoA and Rac1 activation in HMGB-1 stimulated endothelial cells.

Levels of GTP-bound active forms of RhoA and Rac1 as determined by pull-down assays in cultured HAECs 5 to 30 minutes after adding 500 ng/ml HMGB-1. Bars are mean±S.D. of quantitative densitometric analyses from 4 separate experiments. Representative immunoblots from 4 separate experiments are shown. ^* P<0.05 vs. Lane 1.