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. 2014 Dec 9;9(12):e114388. doi: 10.1371/journal.pone.0114388

Figure 4. MyoD overexpression in shSIRT3 restores myoblasts differentiation.

Figure 4

C2C12 and C2C12-SIRT3shRNA (shSIRT3) cells were infected with pBabe empty vector and pBabe-MyoD vector retroviral supernatants. A) Immunostaining with an anti-Troponin T antibody 3 days after the induction of differentiation. Nuclei were stained with Hoechst. Microphotographs of a typical experiment are shown (x400). B) Representative Western-blot of Myogenin at cell confluence (C) and after 3 days of differentiation (D3).