Table 2. ID₅₀ tier-2 pseudotyped virus neutralization titers in the A3R5 assay.

		Env-pseudotyped viruses
Immunogen	Macaque	CAP45	Ce1086	Du151.2	Ce1176_A3	C1080.c03	R2184.c04	SC22.3C2	RHPA
	1	73 #	64	48	44	161	36	218	317
Heterotrimer	2	66	51	52	37	596	53	520	354
RIBI	3	95	104	56	43	418	64	312	373
(Group 1)	4	62	35	43	25	522	40	334	306
	5	145	164	75	46	854	90	1624	347
	Group mean	88	84	55	39	511	57	602	339
	6	20	95	54	39	206	35	470	145
UG37	7	20	52	47	22	204	26	149	61
RIBI	8	20	94	61	33	554	58	356	217
(Group 2)	9	20	72	40	25	991	43	386	147
	10	20	75	71	37	332	57	663	275
	Group mean	20	78	55	31	457	44	405	169
	11	72	69	62	52	1781	44	287	113
UG37	12	147	203	78	48	2822	41	507	459
Carbopol	13	63	219	76	39	929	35	369	215
(Group 3)	14	130	245	77	55	1329	86	509	358
	15	72	387	63	38	433	47	427	146
	Group mean	97	225	71	46	1459	51	420	258
Median $		66	94	61	39	554	44	386	275

^#Numbers given are the sample dilutions at which relative luminescence units (RLU) were reduced by 50% compared to RLU in virus control wells after subtraction of background RLU in cell control wells. All samples were taken two weeks after the third protein boost.

^$Values above the median titer for each pseudovirus are shown in bold.