Fig. 1.

Glomerular detection of autologous cells as source of released chromatin. Kidney sections of lupus prone New Zealand black/white F₁ (NZB/WF₁) mice at different ages (4 and 34 weeks) were stained with anti-Meca32, anti-endoglin (CD105), anti-F480 (all from BioLegend, San Diego, CA, USA) and anti-CD3 (Dako, Glostrup, Denmark). Antibodies were detected by immunohistochemistry using Polink-2 Plus horseradish peroxidase detection kits for tissue (Golden Bridge International, Inc., Mukilteo, WA, USA) according to the protocol described in detail in [50]. All pictures are taken at ×400, scale bar = 50 μm.