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. 2014 Nov 26;179(1):100–107. doi: 10.1111/cei.12436

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Fig. 1 — CD8 T cell clones display differential staining with tetramer loaded with either glutamic acid decarboxylase 9 (GAD9)-mer or GAD10-mer peptide. CD3⁺CD8⁺ clone cells raised against the immunogenic 114–123 region of GAD65 were stained with an irrelevant human leucocyte antigen (HLA)-A*0201 tetramer (loaded with preproinsulin 15–24), GAD9-mer tetramer (GAD_114–122) or GAD10-mer tetramer (GAD_114–123). (a) Clone RK9P9-3 stains with the GAD9-mer tetramer only. (b) Clone RK9C10-1 stains with both GAD9-mer and GAD10-mer tetramers. Histograms are displayed as % of maximum for 10 000 live events, the line indicative of positive staining represents the fluorescence-minus-one control.