Fig. 2.

Exogenous interleukin (IL)-23 does not significantly enhance retinoic acid-related orphan receptor gamma t (RORγt) expression. (a,b) Splenocytes from T cell receptor (TCR) transgenic mice that developed spontaneous experimental autoimmune encephalomyelitis (EAE) were isolated and cultured with myelin basic protein (MBP) Ac1-11, MBP Ac1-11 + interleukin (IL)-23 or MBP Ac1-11 + IL-12 for 72 h. RORγt expression was analysed by flow cytometry (a) and IL-17 production was analysed by enzyme-linked immunosorbent assay (ELISA) (b). (c,d) B6/interferon (IFN)-γ^–/– mice were immunized with myelin oligodendrocyte glycoprotein (MOG) 35–55 emulsified in complete Freund's adjuvant (CFA). Splenocytes were isolated on day 21 after immunization and stimulated with MOG 35–55, MOG 35–55 plus IL-12 or MOG 35–55 plus IL-23 for 3 days. IL-17 production (c) and RORγt expression (d) were analysed by flow cytometry. Cells were gated on CD4⁺ T cells. Data are representative of multiple independent experiments.