Figure 8. Oleoyl-coenzyme A synthetase activity is decreased in the dormancy-induced M. tuberculosis mFATP1-deletion mutant.

Cell-free lysates prepared from Mtb wild type (WT), FACL6-deletion mutant (d-FACL6) and complemented FACL6 mutant (C-FACL6), subjected to dormancy-inducing conditions, were assayed for acyl-CoA synthetase activity as described in Methods. Radioactivity partitioned in aqueous phase after incubation was taken as a measure of enzyme activity. Experiment was repeated independently three times and mean ± SD from duplicates in a representative experiment shown.