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. 2014 Dec 9;9(12):e114877. doi: 10.1371/journal.pone.0114877

Table 2. Metabolic incorporation of 14C-oleic acid-derived radioactivity by Mtb wild-type and d-facl6 mutant.

14C-Oleate Incorporation (DPM)
Total Lipids TAG PL
Log-Phase WT 617600±53740 23850±877 374345±3769
Log-Phase d-FACL6 627200±56144 18400±1146 359285±3288
Dormancy-Induced WT 5225050±466479 2790345±46818 910770±7835
Dormancy-Induced d-FACL6 3910550±253214 1222500±31495 1709215±88579

Mtb wild-type and d-facl6 mutant in log-phase or subjected to dormancy-inducing conditions were incubated with radiolabel as described in Materials and Methods. Radioactivity (DPM, disintegrations per minute) in total lipid extracts was determined by counting dried aliquots by scintillation counting. Radioactivity in triacylglycerol (TAG) and polar lipids (PL) was determined by liquid scintillation counting after TLC separation of total lipid extracts.