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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Curr Protoc Pharmacol. 2014 Dec 1;67:2.11.1–2.11.19. doi: 10.1002/0471141755.ph0211s67

Fig. 3.

Fig. 3

Elution of enhanced truncated cys-less arrestin-1 eVSA-QH-Tr (C63V, C128S, C143A, K257Q, E346H; 1-378; this is bovine cys-less arrestin-1 analogue of the most potent in terms of Rh* binding mouse mutant (Vishnivetskiy et al., 2013)) from heparin-Sepharose. Coomassie gels (left panels) do not allow unambiguous identification of fractions to pool, so it is necessary to rely on Western blots (right panels). M, molecular weight markers; S, protein precipitated by ammonium sulfate and then dissolved and loaded onto heparin-Sepharose; Pt, pass-through; W, wash; fraction numbers are shown (numbers greater than 40 indicate high-salt wash).