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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Curr Protoc Pharmacol. 2014 Dec 1;67:2.11.1–2.11.19. doi: 10.1002/0471141755.ph0211s67

Fig. 4.

Fig. 4

Two-step purification of cys-less arrestin-1 eNCL (C63V, C128S, C143V) (Hanson et al., 2006b; Vishnivetskiy et al., 2010). Coomassie gels for heparin-Sepharose (left panels) and subsequent Q-Sepharose (right panels) are shown. M, molecular weight markers; S, protein precipitated by ammonium sulfate and then dissolved and loaded onto heparin-Sepharose; Pt, pass-through; W, wash; H, pooled fractions from heparin-Sepharose; fraction numbers are shown (numbers greater than 40 after heparin and greater than 30 after Q indicate high-salt wash).