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Western blots of test-expression samples of truncated (1-378; left panel) and full-length (right panel) bovine arrestin-1 mutants on cys-less VSA (C63V, C128S, C143A) background with cysteines in the indicated positions, as compared to eNCL (C63V, C128S, C143V). The blots of supernatant are much more informative than those of homogenate. Note several-fold differences in expression levels, although all proteins express well enough to perform a big prep.
