Table 2.

Purification of WT and mutant arrestins from different species.

	Arrestin	Protein	Elution Peak (NaCl-mM)			Yield (mg/1L of bacterial culture)
Organism			Heparin -Sepharose	Q-Sepharose	SP-Sepharose
Bos taurus	Arrestin-1	WT	190–270	54–80	X	4–5
		1–378	350–450	12–42	-	5
		Cys-less	210–290	34–100	X	8
	Arrestin-2	WT	442–523	97–164	-	13.5
		3A	596–681	150–200	-	3.9
		1–382	623–707	27–80	176–225	7.8
		Cys-less	426–554	120–165	92–145	10
	Arrestin-3	WT	380–500	X	227–327	3.4
		1–382	633–703	17–43	-	0.6
Mus musculus	Arrestin-1	WT	10–55	50–130	-	1.35
		3A	170–300	120–226	-	9.85
Homo sapiens	Arrestin-1	WT	183–289	18–63	X	2.8
	Arrestin-4	WT	10–55	40–130	-	0.4
Ambystoma tigrinum	Arrestin-1	WT	114–341	105–142	-	8.6
		3A	450–590	182–208	-	0.44
	Arrestin-4	WT	90–140	142–165	-	1.4

X, the column is used as a pre-filter to bind impurities and let the arrestin flow through; -, the column is not used.