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Typical yields of total protein at sequential steps of purification (these are achieved by a moderately experienced person; yields can vary +/− 50%)
| Purification step | Protein (mg) | ||
|---|---|---|---|
| Bovine arrestin-1 | Bovine arrestin-2 | Bovine arrestin-3 | |
| Homogenate | ~2,200 | ~2,600 | ~2,900 |
| Supernatant | ~1,100 | ~1,500 | ~1,600 |
| Heparin-Sepharose | ~580 | ~100 | ~30 |
| Q-Sepharose (pure) | ~20 | ~50 | - |
| SP-Sepharose (pure) | - | - | ~13 |
