Extended Data Figure 2.

Properties of designed proteins in this study. a) Sequence alignment of the FFL designs. 3LHP_S is the protein used as the template topology. b) Sequence alignment for the FFL_surf series designed based on the FFL_001 design model. c) Parameters and filtering criteria and results in the design process for FFL designs. ^a SD - standard deviation allowed on the constraints derived from the target topology; ^b Design epitope segment- design of residues within the epitope segment that were not part of the epitope-antibody interface; ^c Filtering criteria based on the helix bend angle; ^d Rosetta energy after human guided optimization. d) Structural diversity in the FFL design models. Values give the backbone RMSD in Å between two designs or between the template (3LHP_S) and the designs. e) Mutational diversity in the FFL designs. Values give the number of mutations between two designs or between the template (3LHP_S) and the designs.