. Author manuscript; available in PMC: 2014 Dec 9.

Published in final edited form as: Nature. 2014 Feb 5;507(7491):201–206. doi: 10.1038/nature12966

Table 1.

Biophysical properties of scaffolds and scaffold-antibody interactions. ND, not done.

Molecule	Multimeric State	T_m (°C)	ΔG (kcal mol^-1)	SPR Motavizumab			NMR-HSQC Dispersion
Molecule	Multimeric State	T_m (°C)	ΔG (kcal mol^-1)	k_on (M^-1s^-1)	k_off (s^-1)	k_off/k_on (pM)	NMR-HSQC Dispersion
FFL_001	Mon	76	ND	3.99 × 10⁶	1.19 × 10^-4	29.9	Dispersed
FFL_002	Mon	49	ND	1.56 × 10⁶	7.34 × 10^-4	469.9	ND
FFL_004	Mon	>85	ND	1.05 × 10⁶	8.32 × 10^-4	795.0	ND
FFL_005	Mon	>100	15.0	2.97 × 10⁶	2.09 × 10^-4	70.3	Partially Dispersed
FFL_006	Mon	>85	ND	3.57 × 10⁶	2.32 × 10^-4	651.9	Dispersed
FFL_007	Mon	>85	ND	1.45 × 10⁶	1.36 × 10^-4	94.1	Partially Dispersed
FFL_001_surf1	Mon	84	8.2	7.43 × 10⁶	4.70 × 10^-4	6.32	ND
FFL_001_surf2	Mon	>85	8.1	5.32 × 10⁶	1.58 × 10^-4	29.6	ND
FFL_001_surf4	Mon	>85	9.0	4.80×10⁶	1.58 × 10^-4	32.9	ND