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. Author manuscript; available in PMC: 2015 Dec 15.
Published in final edited form as: Dev Biol. 2014 Oct 18;396(2):224–236. doi: 10.1016/j.ydbio.2014.10.005

Figure 1. Generation of chick aggrecan transgenic mice.

Figure 1

(A) Schematic representation of the Col2a1-chick aggrecan DNA construct utilized for generation of Agc17/+, Agc18/+, and Agc1283/+ transgenic mice. The construct consists of a 3 kb Col2a1 promoter (hatched box), 237 bp exon 1 (gray box), a 3.02 kb segment of Col2a1 intron 1, and the 6.3 kb chick aggrecan gene. Restriction sites used in cloning are depicted and described in the Materials and Methods section. (B) Representative Southern blot using a 32P-dCTP labeled probe (panel A, red box) of genomic DNA from each of the Agc/+ transgenic lines following XbaI and KpnI digestion (panel A, red). Transgenic line Agc17/+ contained the highest copy number with greater than 50 copies. Representative immunohistochemistry experiments using monoclonal antibodies against chick aggrecan (S103L, C) and chondroitin-6-sulfate (D) are shown for distal femurs of 18.5 dpc embryos of each genotype from the same litter. SA=splice acceptor site